Oreffo R O, Marshall G J, Kirchen M, Garcia C, Gallwitz W E, Chavez J, Mundy G R, Bonewald L F
University of Texas Health Science Center at San Antonio 78284-7877.
Clin Orthop Relat Res. 1993 Nov(296):229-41.
Giant cell tumors of bone are common but unusual tumors that are comprised of multiple cell types. Most attention has been focused on the giant cells, which resemble osteoclasts morphologically and functionally. This study examines the properties of a cell line derived from mononuclear cells from one of these tumors, since it appears likely that these cells may be able to influence the activities of cells with the osteoclast phenotype. This cell line, C433, has the following characteristics: (1) it represents undifferentiated cells, not recognized by any known antigenic markers for leukocytes; (2) it contains tartrate-resistant acid phosphatase; (3) it responds to the osteotropic factors 1,25 dihydroxyvitamin D3, insulin-like growth factor I and II, but not to parathyroid hormone; (4) it forms sarcomas in nude mice; and (5) it produces an activity that stimulates isolated avian and rat osteoclasts to resorb bone. This cell line may be useful in examining interactions between osteoclasts and accessory cells involved in bone resorption.
骨巨细胞瘤是常见但特殊的肿瘤，由多种细胞类型组成。大多数注意力都集中在巨细胞上，这些巨细胞在形态和功能上类似于破骨细胞。本研究检测了源自其中一种肿瘤单核细胞的细胞系的特性，因为这些细胞似乎有可能影响具有破骨细胞表型的细胞的活性。这个细胞系C433具有以下特征：（1）它代表未分化细胞，未被任何已知的白细胞抗原标记物识别；（2）它含有抗酒石酸酸性磷酸酶；（3）它对促骨因子1,25 - 二羟维生素D3、胰岛素样生长因子I和II有反应，但对甲状旁腺激素无反应；（4）它在裸鼠中形成肉瘤；（5）它产生一种活性物质，可刺激分离的禽类和大鼠破骨细胞吸收骨质。该细胞系可能有助于研究破骨细胞与参与骨吸收的辅助细胞之间的相互作用。
