Ikarashi Y, Tsukamoto Y, Tsuchiya T, Nakamura A
National Institute of Hygienic Sciences, Tokyo, Japan.
Contact Dermatitis. 1993 Sep;29(3):128-32. doi: 10.1111/j.1600-0536.1993.tb03509.x.
Dimethyl sulfoxide (DMSO) and sodium lauryl sulfate (SLS) are known to cause irritation of the skin, and to enhance the penetration of chemicals into the epidermis. In the present study, the lymph node cell (LNC) proliferative response following exposure to irritants, such as SLS and DMSO, was examined in the murine local lymph node assay (LLNA). Exposure to DMSO or SLS aqueous solution induced a small increase in lymph node cell proliferation compared with aqueous solution alone. Exposure to SLS in DMSO caused a significant increase in LNC proliferation. Further, the effect of addition of the irritants in a vehicle on the detection of contact sensitivity to metal allergens was examined. Application of potassium dichromate and nickel sulfate in DMSO or SLS aqueous solution caused increases in LNC proliferation. Exposure to metal allergen with SLS in DMSO also induced a significant LNC proliferative response, but did not induce a significant increase in stimulation index (increase in 3H-thymidine incorporation relative to vehicle-treated control group). This was because of increased 3H-thymidine incorporation following exposure to SLS-DMSO in the control group. These results suggest that irritants enhance the LNC proliferative responses to metal allergens. The use of SLS in aqueous solution is effective for the detection of sensitivity to water-soluble allergens, such as metal allergens, in the LLNA, as well as the use of DMSO as an application vehicle.
已知二甲基亚砜（DMSO）和十二烷基硫酸钠（SLS）会引起皮肤刺激，并增强化学物质渗透进入表皮的能力。在本研究中，在小鼠局部淋巴结试验（LLNA）中检测了暴露于刺激物（如SLS和DMSO）后淋巴结细胞（LNC）的增殖反应。与单独的水溶液相比，暴露于DMSO或SLS水溶液会导致淋巴结细胞增殖略有增加。在DMSO中暴露于SLS会导致LNC增殖显著增加。此外，还研究了在赋形剂中添加刺激物对检测金属过敏原接触敏感性的影响。在DMSO或SLS水溶液中应用重铬酸钾和硫酸镍会导致LNC增殖增加。在DMSO中与SLS一起暴露于金属过敏原也会诱导显著的LNC增殖反应，但不会导致刺激指数显著增加（相对于赋形剂处理的对照组，3H-胸腺嘧啶核苷掺入增加）。这是因为对照组在暴露于SLS-DMSO后3H-胸腺嘧啶核苷掺入增加。这些结果表明，刺激物会增强LNC对金属过敏原的增殖反应。在LLNA中，在水溶液中使用SLS对于检测对水溶性过敏原（如金属过敏原）的敏感性是有效的，同时使用DMSO作为应用赋形剂也是有效的。
