Mohimen A, Neumann R, Foster C S, Ahmed A R
Department of Oral Pathology, Harvard School of Dental Medicine, Boston.
Curr Eye Res. 1993 Aug;12(8):741-52. doi: 10.3109/02713689308995770.
Ocular cicatricial pemphigoid (OCP) is a chronic autoimmune inflammatory disease which affects the conjunctiva and other squamous epithelial mucous membranes resulting in a scarring process. It is characterized by the deposition of an anti-basement membrane zone (BMZ) antibody in vivo. Sera from 11 patients with active OCP were studied. Using monkey esophagus and normal skin as substrate, weak staining of the BMZ was observed in conventional indirect immunofluorescence (IIF) assay. Using salt split human skin as substrate, the OCP sera demonstrated binding to the epidermal side of the split, in low titers with weak staining. Ten of the 11 sera were positive on an immunoblot assay using COLO and SCaBER tumor cell lysates demonstrating 230, 205, 160, and 85 kD proteins. Sera from six bullous pemphigoid (BP) patients, with only cutaneous involvement and high titer of anti-BMZ antibody, as detected by IIF, also bound to 230, 160, and 85 kD proteins on both lysates in comigration experiments. Serum from five normal individuals and two patients each with severe atopic conjunctival disease, erythema multiforme with chronic conjunctivitis and systemic lupus erythematosus (SLE), did not demonstrate those bands. When the two lysates were first absorbed with BP sera and then the same lysates were immunoblotted with OCP sera, in all ten OCP sera the 230, 160, and 85 kD bands were eliminated and only a single 205 kD band was uniformly present. These results indicate that OCP sera recognize peptide(s) present in 230, 205 and 160 kD proteins in lysates from COLO and SCaBER tumor cells. These proteins contain the immunodominant region of the BMZ molecule(s) in which the OCP antigen(s) reside. The OCP antigen(s) appears to be distinct from the BP antigen(s).
眼部瘢痕性类天疱疮（OCP）是一种慢性自身免疫性炎症性疾病，可累及结膜和其他鳞状上皮黏膜，导致瘢痕形成过程。其特征是体内抗基底膜带（BMZ）抗体的沉积。对11例活动性OCP患者的血清进行了研究。以猴食管和正常皮肤为底物，在传统间接免疫荧光（IIF）试验中观察到BMZ的弱阳性染色。以盐裂人皮肤为底物，OCP血清显示与裂隙的表皮侧结合，滴度低且染色弱。11份血清中的10份在使用COLO和SCaBER肿瘤细胞裂解物的免疫印迹试验中呈阳性，显示出230、205、160和85 kD的蛋白质。6例大疱性类天疱疮（BP）患者的血清，仅累及皮肤且IIF检测显示抗BMZ抗体滴度高，在共迁移实验中也与两种裂解物上的230、160和85 kD蛋白质结合。5名正常个体以及2例分别患有严重特应性结膜疾病、多形红斑伴慢性结膜炎和系统性红斑狼疮（SLE）患者的血清未显示出这些条带。当两种裂解物先用BP血清吸收，然后用OCP血清对相同的裂解物进行免疫印迹时，在所有10份OCP血清中，230、160和85 kD条带均被消除，仅均匀存在一条205 kD的条带。这些结果表明，OCP血清识别COLO和SCaBER肿瘤细胞裂解物中230、205和160 kD蛋白质中存在的肽段。这些蛋白质包含BMZ分子的免疫显性区域，OCP抗原位于其中。OCP抗原似乎与BP抗原不同。
