Meng X J, Xu Y X, Song X H, Li L, Li H
Institute of Basic Medical Sciences, PAL General Hospital, Beijing.
Chin Med J (Engl). 1993 Jun;106(6):458-62.
Sepsis was induced in rats by cecal ligation and perforation (CLP). Five and 15 hours post CLP, alveolar macrophage (AM), Kupffer cell (KC), and peritoneal macrophage (PM) were isolated and cultured for 18 hours. Culture supernatant was examined for bioactivity of tumor necrosis factor (TNF) and interleukin 6 (IL-6) and response to lipoplysaccharide (LPS) stimulation in vitro. Results showed that AM produced more TNF during sepsis as compared with KC and PM. Stimulation with LPS in vitro was responded only by AM at 15 hr after CLP. Pattern of IL-6 production was different from TNF while KC produced the highest level of IL-6 after induction of sepsis.
通过盲肠结扎穿孔术（CLP）诱导大鼠发生脓毒症。CLP术后5小时和15小时，分离肺泡巨噬细胞（AM）、库普弗细胞（KC）和腹腔巨噬细胞（PM）并培养18小时。检测培养上清液中肿瘤坏死因子（TNF）和白细胞介素6（IL-6）的生物活性以及体外对脂多糖（LPS）刺激的反应。结果显示，与KC和PM相比，脓毒症期间AM产生的TNF更多。CLP术后15小时，体外LPS刺激仅能使AM产生反应。IL-6的产生模式与TNF不同，脓毒症诱导后KC产生的IL-6水平最高。
