Zhou L
People's Hospital, Beijing Medical University.
Zhonghua Wai Ke Za Zhi. 1993 Feb;31(2):79-81.
Hybrid cell line has been derived from fusion of mouse myeloma (Sp2/0-Ag14) cell and spleen cell from mouse which was immunized with cultured cells of giant cell tumor of bone (GCT). Hybrid cells produce monoclonal antibody against GCT associated antigen in the surface of the tumor cells. The monoclonal antibody was detected by immunoperoxidase staining and was designated by GCF-5. There were more than eighty per cent of tested GCT which showed this antigen. It had no cross reaction with many kinds of normal tissue, HLA antigen, alpha-Fetal Protein, Carcino-Embryonic Antigen and beta-Microglobulin. The subclass of the antibody is IgG1. The hybridoma has been cultured and stored under -196 degrees C for more than eight months and its secretion remains stable.
杂交细胞系源自小鼠骨髓瘤（Sp2/0-Ag14）细胞与经骨巨细胞瘤（GCT）培养细胞免疫的小鼠脾细胞的融合。杂交细胞产生针对肿瘤细胞表面GCT相关抗原的单克隆抗体。该单克隆抗体通过免疫过氧化物酶染色检测，并命名为GCF-5。超过80%的受试GCT显示有这种抗原。它与多种正常组织、HLA抗原、甲胎蛋白、癌胚抗原和β-微球蛋白无交叉反应。该抗体的亚类为IgG1。该杂交瘤已在-196℃下培养并保存了八个多月，其分泌仍保持稳定。
