Li Y Y
First Affiliated Hospital of Nanjing Medical College.
Zhonghua Xin Xue Guan Bing Za Zhi. 1993 Feb;21(1):15-7, 62.
Expression of HLA-class II antigens (DQ, DP) in the myocardium of patients with different heart diseases and normal controls was studied with indirect immunofluorescence (IIF). The positive rates in different groups were as follows: dilated cardiomyopathy (DCM, 12/13,+- ), rheumatic heart disease (2/4, +-+ + +), congenital heart diseases (1/14,+), left atrial myxoma (0/1) and normal controls (1/8,+). The immunofluorescent area was in the endocardium and endothelial cells of the blood vessels (VIII factor related antigen positive areas in dual staining of the myocardium). There were significant differences between the positive rates of DCM and those of other heart diseases including rheumatic heart disease, congenital heart diseases and left atrial myxoma (chi 2 cor = 15.3, P < 0.001), as well as between DCM and normal controls (chi 2 cor = 9.9, P < 0.01). The expression of HLA-class II antigens is highly selective. Ectopic expression has been postulated to be responsible for autoimmune diseases. So it can be used as a specific marker for autoimmunity in DCM.
采用间接免疫荧光法（IIF）研究了不同心脏病患者及正常对照者心肌中HLA - II类抗原（DQ、DP）的表达情况。不同组别的阳性率如下：扩张型心肌病（DCM，12/13，±）、风湿性心脏病（2/4，±++++）、先天性心脏病（1/14，+）、左心房黏液瘤（0/1）以及正常对照者（1/8，+）。免疫荧光区域位于心内膜及血管内皮细胞（心肌双重染色中VIII因子相关抗原阳性区域）。DCM的阳性率与包括风湿性心脏病、先天性心脏病和左心房黏液瘤在内的其他心脏病的阳性率之间存在显著差异（校正χ2 = 15.3，P < 0.001），DCM与正常对照者之间也存在显著差异（校正χ2 = 9.9，P < 0.01）。HLA - II类抗原的表达具有高度选择性。据推测，异位表达与自身免疫性疾病有关。因此，它可作为DCM自身免疫的特异性标志物。
