钙和镁与酿酒酵母线粒体无机焦磷酸酶的相互作用。

Interactions of calcium and magnesium with the mitochondrial inorganic pyrophosphatase from Saccharomyces cerevisiae.

作者信息

Uribe S, Rangel P, Pardo J P, Pereira-Da-Silva L

机构信息

Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México.

出版信息

Eur J Biochem. 1993 Oct 15;217(2):657-60. doi: 10.1111/j.1432-1033.1993.tb18289.x.

DOI:10.1111/j.1432-1033.1993.tb18289.x

PMID:8223607

Abstract

The activity of the mitochondrial inorganic pyrophosphatase from Saccharomyces cerevisiae was measured in the presence of increasing concentrations of magnesium and calcium. Calcium pyrophosphate (dissociation constant Kd = 1.9 microM) inhibited pyrophosphatase by competition with magnesium pyrophosphate (Kd = 50 microM). The small movements of calcium detected in mitochondria from yeast may be physiologically significant for the control of inorganic pyrophosphatase activity and the concentration of pyrophosphate in the matrix of yeast mitochondria.

摘要

在不断增加镁和钙浓度的情况下，对酿酒酵母线粒体无机焦磷酸酶的活性进行了测定。焦磷酸钙（解离常数Kd = 1.9微摩尔）通过与焦磷酸镁（Kd = 50微摩尔）竞争来抑制焦磷酸酶。在酵母线粒体中检测到的钙的微小变化，可能在生理上对控制无机焦磷酸酶活性以及酵母线粒体基质中焦磷酸的浓度具有重要意义。