Guiso N, Grimprel E, Anjak I, Bégué P
Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France.
Eur J Clin Microbiol Infect Dis. 1993 Aug;12(8):596-600. doi: 10.1007/BF01973637.
Western blot and agglutination techniques were used to analyze the antibody responses to Bordetella pertussis in 27 infants less than six month of age with presumed pertussis infection. The antibody response to the Bordetella pertussis adhesions filamentous hemagglutinin, pertactin and agglutinogens, and to the Bordetella pertussis toxins pertussis toxin and adenylate cyclase-hemolysin were compared. Infection induced intense antibody responses to filamentous hemagglutinin, pertussis toxin and adenylate cyclase-hemolysin. Antibodies to agglutinogens were never detected, and antibodies to pertactin were rarely detected in infected infants' sera. Therefore, determination of anti-agglutinogens levels only is not suitable for the serological diagnosis of pertussis in young infants. Use of purified filamentous hemagglutinin, pertussis toxin and adenylate cyclase-hemolysin in Western blot analysis may improve the serodiagnosis of Bordetella infections. However, care must be exercised in distinguishing between the antibody response in young infants and maternally derived antibodies.
采用蛋白质印迹法和凝集技术，对27名6月龄以下疑似感染百日咳博德特氏菌的婴儿的百日咳抗体反应进行分析。比较了对百日咳博德特氏菌黏附素丝状血凝素、百日咳杆菌黏附素和凝集原，以及对百日咳博德特氏菌毒素百日咳毒素和腺苷酸环化酶溶血素的抗体反应。感染引发了对丝状血凝素、百日咳毒素和腺苷酸环化酶溶血素的强烈抗体反应。在受感染婴儿的血清中从未检测到凝集原抗体，且很少检测到百日咳杆菌黏附素抗体。因此，仅测定抗凝集原水平不适用于幼儿百日咳的血清学诊断。在蛋白质印迹分析中使用纯化的丝状血凝素、百日咳毒素和腺苷酸环化酶溶血素可能会改善博德特氏菌感染的血清学诊断。然而，在区分幼儿的抗体反应和母源抗体时必须谨慎。
