Gourdji D, Tixier-Vidal A, Levine-Pinto H, Pradelles P, Morgat J L, Fromageot P
Neuroendocrinology. 1976;20(3):201-11. doi: 10.1159/000122484.
GH3 cells which had been exposed for 30 min to (3H)-TRH and had performed their maximun biological response, i.e. increase of prolactin (PRL) release, retained intracellular radioactivity which consisted of chemically unmodified TRH (greater than or equal to 93%). Such preloaded GH3 cells were found able to spontaneously release into the culture medium a radioactive material (3H)-RM) which was analyzed. The kinetics of binding of (3H)-RM to intact GH3 cells and competition with unlabeled TRH were indistinguishable from (3H-TRH. (3H)-RM was able to stimulate PRL release from GH3 cells. In addition thin layer electrophoresis (TLE) revelaed that 90% of (3H)-RM migrated like TRH reference. All these features strongly suggest that (3H)-RM is identical to (3H)-TRH.
已暴露于（3H）-促甲状腺激素释放激素（TRH）30分钟并表现出最大生物学反应（即催乳素（PRL）释放增加）的GH3细胞，保留了由化学未修饰的TRH（大于或等于93%）组成的细胞内放射性。发现这种预先加载的GH3细胞能够自发地将一种放射性物质（3H）-RM释放到培养基中，并对其进行了分析。（3H）-RM与完整GH3细胞的结合动力学以及与未标记TRH的竞争与（3H）-TRH无法区分。（3H）-RM能够刺激GH3细胞释放PRL。此外，薄层电泳（TLE）显示90%的（3H）-RM迁移情况与TRH对照相同。所有这些特征都强烈表明（3H）-RM与（3H）-TRH相同。
