Gourdji D, Tixier-Vidal A, Levine-Pinto H, Pradelles P H, Morgat J L, Fromageot P
Ann Endocrinol (Paris). 1976 Mar-Apr;37(2):119-20.
It was previously shown that after a 30 min. incubation with [3H]-TRH intact GH3 cells have already performed their optimal precocious biological response to TRH (i.e increase of prolactin release) and that chemically unmodified ( greater than or equal to 93%) [3H]-TRH entered the cells and remained associated to them. The present work demonstrates that such cells spontaneously release a radioactive material ([3H]RM) when post-incubated at 37 degrees C. The binding to GH3 cells (time course and competition with unlabelled TRH) as well as the biological effect on prolactin release and the electrophoretic mobility of the [3H]-RM released in one hour were compared to those of [3H]-TRH. According to those criteria [3H]-RM is undistinguishable from [3H]-TRH at 90%. Our data, therefore, support the hypothesis of a continuous flow of TRH molecules on both sides of the plasma membrane during TRH-GH3 cells interaction.
先前的研究表明，在与[3H]-促甲状腺激素释放激素（TRH）孵育30分钟后，完整的GH3细胞已经对TRH产生了最佳的早熟生物学反应（即催乳素释放增加），并且化学未修饰（≥93%）的[3H]-TRH进入细胞并与它们保持结合。目前的研究表明，当在37℃下进行后孵育时，这些细胞会自发释放一种放射性物质（[3H]RM）。将[3H]RM与GH3细胞的结合（时间进程以及与未标记TRH的竞争）、对催乳素释放的生物学效应以及一小时内释放的[3H]RM的电泳迁移率与[3H]-TRH进行了比较。根据这些标准，[3H]RM在90%的程度上与[3H]-TRH无法区分。因此，我们的数据支持了在TRH与GH3细胞相互作用期间，TRH分子在质膜两侧持续流动的假说。
