Rutten T L, Knuiman B
Department of Experimental Botany, University of Nijmegen, The Netherlands.
Eur J Cell Biol. 1993 Aug;61(2):247-55.
Pollen germination and pollen tube growth were clearly affected by the drug brefeldin A: both processes could be stopped completely. Notable thickening of the cell wall was not observed in non-growing pollen tubes, and plasma streaming remained vigorous in the presence of brefeldin A. Over time, the tip region became filled with a dense cytoplasm that lacked large organelles. Ultrastructural observation showed the rapid dissociation and disappearance of Golgi bodies. The concomitant appearance of vesicle-like structures attached to the endoplasmic reticulum (ER) suggests a fusion of the Golgi with the ER similar to that which happens in animal cells. Secretory vesicles disappeared from drug-treated tubes, and the tip region became filled with tubular ER. As the actions of the drug seemed to be reversible, brefeldin A may contribute substantially to research on Golgi dynamics and tip growth in pollen tubes.
布雷菲德菌素A明显影响花粉萌发和花粉管生长：这两个过程都可能完全停止。在不生长的花粉管中未观察到细胞壁明显增厚，并且在布雷菲德菌素A存在的情况下胞质环流仍然活跃。随着时间的推移，顶端区域充满了缺乏大型细胞器的致密细胞质。超微结构观察显示高尔基体迅速解离并消失。附着在内质网上的囊泡状结构的同时出现表明高尔基体与内质网融合，类似于在动物细胞中发生的情况。分泌囊泡从药物处理的花粉管中消失，顶端区域充满了管状内质网。由于药物的作用似乎是可逆的，布雷菲德菌素A可能对花粉管中高尔基体动力学和顶端生长的研究有很大贡献。
