Rasmussen S N, Iversen P
Pflugers Arch. 1976 Jun 22;363(3):239-44. doi: 10.1007/BF00594608.
The extravascular pool of albumin in the non-diuretic rat kidney was determined as the difference between total and intravascular albumin pools. The total pool of albumin was determined in two ways in the same kideny: 1. as the distribution volume of rat endogenous albumin measured immunochemically and 2. as the distribution volume of 125I-labelled rat albumin equilibrated for 4 days. The distribution volume of 131I human albumin after 1 min equilibration was taken as a measure of the intravascular pool. Total albumin pool estimated as rat endogenous albumin volume of distribution averaged 13.4, 25.1, and 43.0 mul per 100 mg tissue in cortex, outer and inner medulla, respectively. Similar figures were obtained using 125I-labelled rat albumin. Intravascular pool of albumin in the 3 zones averaged 7.2, 19.7, and 26.8 mul per 100 mg tissue. The calculated extravascular fraction of albumin averaged 0.44, 0.21, and 0.35 for cortex, outer and inner medulla, respectively.
非利尿大鼠肾脏中白蛋白的血管外池被确定为总白蛋白池与血管内白蛋白池之间的差值。在同一肾脏中，总白蛋白池通过两种方法测定：1. 作为通过免疫化学方法测定的大鼠内源性白蛋白的分布容积；2. 作为经4天平衡后的125I标记大鼠白蛋白的分布容积。平衡1分钟后131I人白蛋白的分布容积被用作血管内池的指标。以大鼠内源性白蛋白分布容积估算的总白蛋白池在皮质、外髓和内髓中分别平均为每100mg组织13.4、25.1和43.0μl。使用125I标记大鼠白蛋白也得到了类似的数据。三个区域中白蛋白的血管内池平均为每100mg组织7.2、19.7和26.8μl。皮质、外髓和内髓中计算得出的白蛋白血管外分数分别平均为0.44、0.21和0.35。
