Boisjoly H M, Laplante C, Bernatchez S F, Salesse C, Giasson M, Joly M C
Ophthalmology Research Unit, C.H.U.L. Research Center, Laval University, Québec, Canada.
Exp Eye Res. 1993 Sep;57(3):293-300. doi: 10.1006/exer.1993.1127.
We investigated the effects of EGF, IL-1 and their combination on closure of wounds inflicted on rabbit corneal epithelial cell cultures and on migration of these cells in microchemotaxis chambers. In vitro corneal epithelial wound closure depended on the applied concentrations of EGF or IL-1. Twenty-four hours after wounding, the smallest wounds were obtained with 50 ng ml-1 of EGF and 1 ng ml-1 of IL-1, respectively. The effect on wound closure of combinations of EGF and IL-1 was additive even at concentrations that were optimal for each growth factor when applied alone. We found that EGF increases the chemotactic migration of rabbit corneal epithelial cells. Cell chemotaxis depended both on the concentration of EGF and on the number of cells applied in the assay. This response to EGF was seen at concentrations that were effective in the wound closure assay. The magnitude of the chemotactic migration response was much smaller with IL-1 than with EGF. Similarly to the observations on wound closure, the effect on cell chemotaxis of combinations of EGF and IL-1 was additive. The ability of EGF, and EGF/IL-1 combinations to modulate corneal epithelial cell chemotactic migration supports migration as a possible biological mechanism of the acceleration of corneal epithelium wound closure by these drugs.
我们研究了表皮生长因子（EGF）、白细胞介素-1（IL-1）及其组合对兔角膜上皮细胞培养物伤口愈合以及这些细胞在微趋化室中迁移的影响。体外角膜上皮伤口愈合取决于所应用的EGF或IL-1浓度。受伤24小时后，分别用50 ng/ml的EGF和1 ng/ml的IL-1可使伤口最小。即使在单独应用时对每种生长因子而言都是最佳的浓度下，EGF和IL-1组合对伤口愈合的影响也是相加的。我们发现EGF可增加兔角膜上皮细胞的趋化性迁移。细胞趋化性既取决于EGF的浓度，也取决于测定中所应用的细胞数量。在伤口愈合测定中有效的浓度下可观察到对EGF的这种反应。IL-1引起的趋化性迁移反应程度比EGF小得多。与伤口愈合的观察结果相似，EGF和IL-1组合对细胞趋化性的影响是相加的。EGF以及EGF/IL-1组合调节角膜上皮细胞趋化性迁移的能力支持迁移是这些药物加速角膜上皮伤口愈合的一种可能生物学机制。
