Sumner M T
Department of Chemistry, University of North Carolina at Chapel Hill 27599-3290.
FEBS Lett. 1993 Oct 25;333(1-2):35-8. doi: 10.1016/0014-5793(93)80370-a.
The binding of five different mouse monoclonal anti-dinitrophenyl (DNP) IgGl monomers, in the presence and absence of DNP-glycine, to Fc gamma RII on the mouse macrophage-like cell line J774 has been investigated. Membrane association constants were estimated using a competitive radioimmunoassay in which the ability of the IgG1 monomers to block the binding of 125I-labelled Fabs of the anti-Fc gamma RII monoclonal antibody 2.4G2 was monitored. The IgG1-Fc gamma RII association constants ranged from (8 +/- 1) x 10(4) M-1 to (6 +/- 2) x 10(5) M-1. No large differences in the IgG1-Fc gamma RII association constants were measured for different IgG1 antibodies or for any single IgG1 antibody in the presence and absence of DNP-glycine.
研究了五种不同的小鼠单克隆抗二硝基苯基（DNP）IgG1单体在存在和不存在DNP-甘氨酸的情况下与小鼠巨噬细胞样细胞系J774上的FcγRII的结合。使用竞争性放射免疫测定法估计膜结合常数，其中监测IgG1单体阻断抗FcγRII单克隆抗体2.4G2的125I标记Fabs结合的能力。IgG1-FcγRII结合常数范围为(8±1)×10(4) M-1至(6±2)×10(5) M-1。对于不同的IgG1抗体或任何单一IgG1抗体，在存在和不存在DNP-甘氨酸的情况下，未测量到IgG1-FcγRII结合常数有很大差异。
