Schalkwijk C G, Vervoordeldonk M, Pfeilschifter J, van den Bosch H
Centre for Biomembranes and Lipid Enzymology, Utrecht, The Netherlands.
FEBS Lett. 1993 Nov 1;333(3):339-43. doi: 10.1016/0014-5793(93)80683-l.
Interleukin-1 beta induces gene expression and secretion of the secretory phospholipase A2 (sPLA2) and prostaglandin E2 (PGE2) release from rat mesangial cells. We have previously shown that prolonged treatment of rat mesangial cells with interleukin-1 beta (IL-1 beta) also enhances the cytosolic phospholipase A2 (cPLA2) activity. This effect of IL-1 beta on the cPLA2 activity is inhibited by actinomycin D and cycloheximide, indicating that both transcription and translation are involved. Here, we describe that IL-1 beta increases mRNA levels and protein synthesis of cPLA2 itself. In parallel with the effect of dexamethasone on the sPLA2, this glucocorticoid inhibits the IL-1 beta-enhanced cPLA2 activity as a result of the suppression of IL-1 beta-induced cPLA2 gene expression. This report suggests that the pro-inflammatory action of interleukin-1 beta may, in part, be mediated by its effects on cPLA2 activity.
白细胞介素-1β可诱导大鼠系膜细胞中分泌型磷脂酶A2（sPLA2）的基因表达和分泌以及前列腺素E2（PGE2）的释放。我们之前已经表明，用白细胞介素-1β（IL-1β）长时间处理大鼠系膜细胞也会增强胞质型磷脂酶A2（cPLA2）的活性。IL-1β对cPLA2活性的这种作用被放线菌素D和环己酰亚胺抑制，这表明转录和翻译均参与其中。在此，我们描述IL-1β会增加cPLA2自身的mRNA水平和蛋白质合成。与地塞米松对sPLA2的作用类似，这种糖皮质激素由于抑制了IL-1β诱导的cPLA2基因表达，从而抑制了IL-1β增强的cPLA2活性。本报告表明，白细胞介素-1β的促炎作用可能部分是由其对cPLA2活性的影响介导的。
