鸟嘌呤核苷酸对艾美球虫腺苷酸环化酶的调节作用。
Regulation of adenylyl cyclase from Blastocladiella emersonii by guanine nucleotides.
作者信息
Terenzi H, Maia J C
机构信息
Departamento de Bioquímica, Universidade de São Paulo, Brazil.
出版信息
FEBS Lett. 1993 Nov 8;334(1):9-12. doi: 10.1016/0014-5793(93)81669-q.
GTP gamma S stimulates adenylyl cyclase in particulate fractions of Blastocladiella emersonii zoospores. Cholera toxin catalyses the ADP-ribosylation of a membrane protein of a molecular weight (46,000) similar to that of the alpha subunit of Gs found in vertebrate cells. A membrane protein of 46 kDa can also be recognized in Western blots by an antipeptide antiserum (RM/1) raised against the C-terminus of G alpha 2-subunits. These results suggest that a G-protein mediates the regulation of Blastocladiella adenylyl cyclase by guanine nucleotides.
鸟苷三磷酸γ-硫酯（GTPγS）可刺激艾美球囊霉游动孢子微粒体部分中的腺苷酸环化酶。霍乱毒素催化一种分子量为46,000的膜蛋白的ADP核糖基化，该分子量与脊椎动物细胞中发现的Gsα亚基相似。在蛋白质免疫印迹中，一种针对Gα2亚基C末端产生的抗肽抗血清（RM/1）也能识别出一种46 kDa的膜蛋白。这些结果表明，一种G蛋白通过鸟嘌呤核苷酸介导对艾美球囊霉腺苷酸环化酶的调节。
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