Liu H C, Cohen J, Alikani M, Noyes N, Rosenwaks Z
Department of Obstetrics and Gynecology, New York Hospital-Cornell Medical Center.
Fertil Steril. 1993 Nov;60(5):871-5.
To examine whether opening of the zona pellucida (i.e., assisted hatching) accelerates implantation.
In a controlled, randomized trial, patients were assigned to control and assisted hatching groups.
All patients studied were of the Center for Reproductive Medicine at Cornell University Medical College.
All patients underwent stimulation with gonadotropins after luteal phase GnRH down regulation. Assisted hatching with zona drilling using acidic Tyrode's solution was performed on the assigned embryos.
Luteal E2, P, and hCG on days +5, +6, +7, +8, +9, +11, +13, and +15 were measured. The implantation time, peak midluteal E2 and intervals between these two values were studied.
Implantation occurred significantly earlier in the assisted hatching group. The interval between implantation and peak midluteal E2 was also significantly shorter in the assisted hatching group than in the controls. However, there was no significant difference in the day of the peak midluteal E2 between the assisted hatching and control groups.
Assisted hatching may enhance embryo implantation not only by mechanically facilitating the hatching process but also by allowing earlier embryo-endometrium contact. Such early contact may enhance embryonic development potential and may optimize synchronization between embryo and endometrium, resulting in improved implantation efficiency.
研究透明带开口（即辅助孵化）是否能加速着床。
在一项对照随机试验中，将患者分为对照组和辅助孵化组。
所有研究患者均来自康奈尔大学医学院生殖医学中心。
所有患者在黄体期促性腺激素释放激素（GnRH）下调后接受促性腺激素刺激。对分配的胚胎使用酸性台氏液进行透明带钻孔辅助孵化。
测量黄体期第5、6、7、8、9、11、13和15天的E2、P和hCG。研究着床时间、黄体中期E2峰值以及这两个值之间的间隔。
辅助孵化组着床明显更早。辅助孵化组着床与黄体中期E2峰值之间的间隔也明显短于对照组。然而，辅助孵化组和对照组黄体中期E2峰值出现的日期没有显著差异。
辅助孵化不仅可以通过机械方式促进孵化过程，还可以通过使胚胎与子宫内膜更早接触来增强胚胎着床。这种早期接触可能会增强胚胎发育潜力，并可能优化胚胎与子宫内膜之间的同步性，从而提高着床效率。
