使用带荧光检测的高效液相色谱法测定大西洋鲑（Salmo salar）组织中的伊维菌素残留量。

Determination of ivermectin residues in the tissues of Atlantic salmon (Salmo salar) using HPLC with fluorescence detection.

作者信息

Kennedy D G, Cannavan A, Hewitt S A, Rice D A, Blanchflower W J

机构信息

Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast, UK.

出版信息

Food Addit Contam. 1993 Sep-Oct;10(5):579-84. doi: 10.1080/02652039309374181.

DOI:10.1080/02652039309374181

PMID:8224326

Abstract

A sensitive, accurate and reproducible assay for the determination of residual concentrations of ivermectin in the tissues of farmed Atlantic salmon is described. The assay employs the clean-up of an acetonitrile extract of salmon tissue on a Bond-Elut C8 column, followed by derivatization with trifluoroacetic acid anhydride. Following separation using HPLC, 22,23-dihydroavermectin B1a is determined using fluorescence detection. The limit of detection of the assay is 1 ng per g tissue. Liver, taken from fish treated experimentally with ivermectin, was found to contain higher concentrations of 22,23-dihydroavermectin B1a than any other tissue examined. The half-life of 22,23-dihydroavermectin B1a was similar in all tissues studied, ranging from 89 to 98 degree days.

摘要

本文描述了一种灵敏、准确且可重复的方法，用于测定养殖大西洋鲑鱼组织中伊维菌素的残留浓度。该方法采用在Bond-Elut C8柱上对鲑鱼组织的乙腈提取物进行净化，然后用三氟乙酸酐进行衍生化。使用高效液相色谱法分离后，通过荧光检测测定22,23-二氢阿维菌素B1a。该测定方法的检测限为每克组织1纳克。从经伊维菌素实验处理的鱼中取出的肝脏，被发现含有比其他任何检测组织更高浓度的22,23-二氢阿维菌素B1a。在所有研究的组织中，22,23-二氢阿维菌素B1a的半衰期相似，范围为89至98度日。