人表皮和肝脏中使环氧化物水合的酶的特性。

Properties of enzymes hydrating epoxides in human epidermis and liver.

Winder B S, Nourooz-Zadeh J, Isseroff R R, Moghaddam M F, Hammock B D

University College and Middlesex School of Medicine, London, U.K.

Int J Biochem. 1993 Sep;25(9):1291-301. doi: 10.1016/0020-711x(93)90081-o.

Abstract

Cytosolic and microsomal epoxide hydrolyzing enzymes of human skin and liver were compared and found to be different. 2. Epidermal and hepatic cytosolic epoxide hydrolases were different in terms of substrate selectivity, pI, inhibitor sensitivity and affinity chromatographic properties. 3. Microsomal epoxide hydrolases had the same pIs but different substrate selectivities. 4. Cytosolic epoxide hydrolase from adults had higher specific activity than that from neonates or cultured epidermis, but lower activity than adult hepatic enzymes. 5. The sizes of cytosolic epoxide hydrolase from epidermis and liver were similar and lower than that from cultured fibroblasts. 6. Cytosolic epoxide hydrolase from all sources shared similar antigenic determinants.

摘要

对人皮肤和肝脏的胞质和微粒体环氧化物水解酶进行了比较，发现二者有所不同。2. 表皮和肝脏的胞质环氧化物水解酶在底物选择性、等电点、抑制剂敏感性和亲和色谱特性方面存在差异。3. 微粒体环氧化物水解酶具有相同的等电点，但底物选择性不同。4. 成人的胞质环氧化物水解酶比新生儿或培养的表皮中的酶具有更高的比活性，但比成人肝脏酶的活性低。5. 表皮和肝脏的胞质环氧化物水解酶的大小相似，且低于培养的成纤维细胞中的酶。6. 所有来源的胞质环氧化物水解酶具有相似的抗原决定簇。