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Purification schemes for the constituent domains of cytochrome P450 BM3 in E. coli.

作者信息

Munro A W

机构信息

Department of Biochemistry, University of Glasgow.

出版信息

Biochem Soc Trans. 1993 Aug;21 ( Pt 3)(3):316S. doi: 10.1042/bst021316s.

DOI:10.1042/bst021316s
PMID:8224461
Abstract
摘要

相似文献

1
Purification schemes for the constituent domains of cytochrome P450 BM3 in E. coli.大肠杆菌中细胞色素P450 BM3组成结构域的纯化方案。
Biochem Soc Trans. 1993 Aug;21 ( Pt 3)(3):316S. doi: 10.1042/bst021316s.
2
Purification of cytochrome P450 BM-3 as a monooxygenase.作为单加氧酶的细胞色素P450 BM-3的纯化
Protein Pept Lett. 2005 May;12(4):327-31. doi: 10.2174/0929866053765653.
3
Purification of functional recombinant P450s from bacteria.
Methods Enzymol. 1996;272:35-44. doi: 10.1016/s0076-6879(96)72006-2.
4
Expression, purification, and characterization of Bacillus subtilis cytochromes P450 CYP102A2 and CYP102A3: flavocytochrome homologues of P450 BM3 from Bacillus megaterium.枯草芽孢杆菌细胞色素P450 CYP102A2和CYP102A3的表达、纯化及特性分析:巨大芽孢杆菌P450 BM3的黄素细胞色素同源物
Biochemistry. 2004 May 11;43(18):5474-87. doi: 10.1021/bi035904m.
5
A novel inhibitor of cytochrome P450 BM3.一种新型细胞色素P450 BM3抑制剂。
Biochem Soc Trans. 1993 Nov;21(4):411S. doi: 10.1042/bst021411s.
6
Optimization of fermentation conditions for P450 BM-3 monooxygenase production by hybrid design methodology.采用混合设计方法优化用于生产P450 BM-3单加氧酶的发酵条件。
J Zhejiang Univ Sci B. 2007 Jan;8(1):27-32. doi: 10.1631/jzus.2007.B0027.
7
Probing electron transfer in flavocytochrome P-450 BM3 and its component domains.探究黄素细胞色素P-450 BM3及其组成结构域中的电子转移
Eur J Biochem. 1996 Jul 15;239(2):403-9. doi: 10.1111/j.1432-1033.1996.0403u.x.
8
Construction of a human cytochrome P450 1A1: rat NADPH-cytochrome P450 reductase fusion protein cDNA and expression in Escherichia coli, purification, and catalytic properties of the enzyme in bacterial cells and after purification.人细胞色素P450 1A1:大鼠NADPH-细胞色素P450还原酶融合蛋白cDNA的构建及其在大肠杆菌中的表达、纯化,以及该酶在细菌细胞内和纯化后的催化特性
Arch Biochem Biophys. 1996 Jun 1;330(1):48-58. doi: 10.1006/abbi.1996.0224.
9
Regional saturation mutagenesis as an approach to identification of substrate specificity determinants in cytochrome P450 BM3.
Biochem Soc Trans. 1993 Nov;21(4):409S. doi: 10.1042/bst021409s.
10
Development of a fed-batch process for the production of the cytochrome P450 monooxygenase CYP102A1 from Bacillus megaterium in E. coli.开发一种补料分批培养工艺,用于在大肠杆菌中生产来自巨大芽孢杆菌的细胞色素P450单加氧酶CYP102A1。
J Biotechnol. 2007 May 1;129(3):481-8. doi: 10.1016/j.jbiotec.2007.01.013. Epub 2007 Jan 30.

引用本文的文献

1
The role of tryptophan 97 of cytochrome P450 BM3 from Bacillus megaterium in catalytic function. Evidence against the 'covalent switching' hypothesis of P-450 electron transfer.巨大芽孢杆菌细胞色素P450 BM3中色氨酸97在催化功能中的作用。对P-450电子转移“共价转换”假说的反驳证据。
Biochem J. 1994 Oct 15;303 ( Pt 2)(Pt 2):423-8. doi: 10.1042/bj3030423.