Brooks J, Taylor P L, Saunders P T, Eidne K A, Struthers W J, McNeilly A S
MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, UK.
Mol Cell Endocrinol. 1993 Aug;94(2):R23-7. doi: 10.1016/0303-7207(93)90177-l.
We have isolated a full length cDNA clone coding for the sheep GnRH receptor (GnRH-R). The amino acid sequence shows greater homology to the human GnRH-R sequence than the two rodent receptors published so far. We have also carried out physiological studies investigating the pattern of expression of the GnRH-R mRNA throughout the estrous cycle. GnRH receptor mRNA and GnRH binding levels were both significantly (P < 0.05) increased over luteal levels up until the time of the preovulatory LH surge, whilst post-surge, a significant (P < 0.05) decline was seen. These changes were related to increased follicle estradiol production in the follicular phase. In contrast, no changes in the abundance of LH beta mRNA were seen throughout the estrous cycle, but the pituitary content of LH significantly (P < 0.05) decreased after the preovulatory LH surge. These results suggest that there is a close relationship between the abundance of GnRH-R mRNA and translation of the GnRH-R in sheep.
我们分离出了一个编码绵羊促性腺激素释放激素受体（GnRH-R）的全长cDNA克隆。与迄今为止已发表的两种啮齿动物受体相比，该氨基酸序列与人类GnRH-R序列具有更高的同源性。我们还进行了生理学研究，调查了整个发情周期中GnRH-R mRNA的表达模式。直到排卵前促黄体生成素（LH）激增时，GnRH受体mRNA和GnRH结合水平均比黄体期水平显著升高（P < 0.05），而在激增后则出现显著下降（P < 0.05）。这些变化与卵泡期卵泡雌二醇产量增加有关。相比之下，在整个发情周期中未观察到LHβ mRNA丰度的变化，但在排卵前LH激增后，垂体中LH的含量显著降低（P < 0.05）。这些结果表明，绵羊中GnRH-R mRNA的丰度与GnRH-R的翻译之间存在密切关系。
