Zimmerman W, Weijer C J
Zoologisches Institut, Universität München, Germany.
Dev Biol. 1993 Nov;160(1):178-85. doi: 10.1006/dbio.1993.1296.
Labeling with the thymidine analog 5'-bromodeoxyuridine has been used to follow cell cycling during development of the cellular slime mould Dictyostelium discoideum. The results show that cycling continues during development and that 75% of all cells from axenically grown cultures go through S-phase. Although cell cycle progression is temporarily blocked in all cells during early aggregation, it is specifically reinitiated in prespore cells later in development. Prestalk cells do not reenter the cycle. Pulse chase experiments indicate a close correlation between cell cycle phase at the onset of development and differentiation fate. Cells in S-phase and early G2 differentiate preferentially into prestalk cells; cells in the later part of G2 differentiate into prespore cells.
用胸苷类似物5'-溴脱氧尿苷进行标记，已被用于追踪细胞黏菌盘基网柄菌发育过程中的细胞周期。结果表明，发育过程中细胞周期持续进行，且来自无菌培养物的所有细胞中有75%经历S期。尽管在早期聚集过程中所有细胞的细胞周期进程暂时受阻，但在发育后期它会在芽孢前体细胞中特异性地重新启动。柄细胞前体不会重新进入细胞周期。脉冲追踪实验表明，发育开始时的细胞周期阶段与分化命运之间密切相关。处于S期和G2早期的细胞优先分化为柄细胞前体；处于G2后期的细胞分化为芽孢前体细胞。
