Cerdá J L, Petrino T R, Wallace R A
Instituto de Acuicultura Torre de la Sal (CSIC), Castellón, Spain.
Dev Biol. 1993 Nov;160(1):228-35. doi: 10.1006/dbio.1993.1300.
The physiological significance of heterologous gap junctions between granulosa cells and the oocyte was investigated in late vitellogenic ovarian follicles of the teleost Fundulus heteroclitus. Lucifer Yellow injected into the oocyte readily passed to the overlying granulosa cells, demonstrating effective dye-coupling. Passage of the fluorescent dye, and hence intercellular communication, was inhibited both by the tumor-promoting phorbol ester phorbol 12-myristate 13-acetate (PMA) and by 1-octanol, known uncouplers of gap junctions in a variety of invertebrate and vertebrate cell types. Octanol alone also initiated resumption of meiosis in follicle-enclosed oocytes, indicating that granulosa cells normally maintain meiotic arrest, as apparently occurs in mammalian and amphibian follicles. Both PMA and octanol also consistently inhibited the hydration process that normally accompanies meiotic maturation. These results support a previously suggested hypothesis that K+, which is the primary osmotic effector for oocyte hydration, is translocated via gap junction from granulosa cells to the maturing oocyte.
在底鳉（Fundulus heteroclitus）的晚卵黄生成期卵巢卵泡中，研究了颗粒细胞与卵母细胞之间异源间隙连接的生理意义。注入卵母细胞的荧光黄很容易传递到覆盖其上的颗粒细胞，表明存在有效的染料偶联。荧光染料的传递以及细胞间通讯均受到促肿瘤佛波酯佛波醇12 -肉豆蔻酸酯-13 -乙酸酯（PMA）和1 -辛醇的抑制，这两种物质是多种无脊椎动物和脊椎动物细胞类型中已知的间隙连接解偶联剂。单独的辛醇也会引发卵泡包被的卵母细胞减数分裂的恢复，这表明颗粒细胞通常维持减数分裂停滞，这在哺乳动物和两栖动物卵泡中显然也会发生。PMA和辛醇也都持续抑制通常伴随减数分裂成熟的水化过程。这些结果支持了之前提出的一个假设，即钾离子作为卵母细胞水化的主要渗透效应物，通过间隙连接从颗粒细胞转运到成熟的卵母细胞。
