Chambers C, Russell P
Section on Cataracts, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
Gene. 1993 Nov 15;133(2):295-9. doi: 10.1016/0378-1119(93)90655-m.
This study reports the nucleotide (nt) sequence of the human beta B2-crystallin (h beta B2-Cry)-encoding cDNA (h beta B2-cry). The h beta B2-cry gene encodes a major structural protein in the lens of the vertebrate eye. Sequence information obtained from mouse genomic clones and the mouse beta B2-cry cDNA (m beta B2-cry) sequence was used to design oligodeoxyribonucleotide primers, corresponding to exon 1, transcription start points, and termination and polyadenylation sites, that were used in the polymerase chain reaction (PCR) to generate full-length cDNA clones from total human lens RNA. In addition, cDNA libraries were made with lambda gt11 from both human fetal and adult lens tissue RNAs, and screened with a m beta B2-cry cDNA clone. The full-length h beta B2-cry cDNA is 721 bp and contains an open reading frame of 612 nt. It codes for a 23-kDa protein of 205 amino acid residues. Comparison of the overall nt and deduced aa sequences shows a greater similarity of h beta B2-cry to bovine beta B2-cry than to m beta B2-cry or rat beta B2-cry.
本研究报告了人类βB2-晶状体蛋白（hβB2-Cry）编码cDNA（hβB2-cry）的核苷酸（nt）序列。hβB2-cry基因编码脊椎动物眼睛晶状体中的一种主要结构蛋白。从小鼠基因组克隆获得的序列信息以及小鼠βB2-cry cDNA（mβB2-cry）序列被用于设计寡脱氧核糖核苷酸引物，这些引物对应于外显子1、转录起始点、终止和聚腺苷酸化位点，用于聚合酶链反应（PCR），以从人晶状体总RNA中生成全长cDNA克隆。此外，用人胎儿和成人晶状体组织RNA构建了λgt11 cDNA文库，并用mβB2-cry cDNA克隆进行筛选。全长hβB2-cry cDNA为721 bp，包含一个612 nt的开放阅读框。它编码一个由205个氨基酸残基组成的23 kDa蛋白质。整体nt序列和推导的氨基酸序列比较表明，hβB2-cry与牛βB2-cry的相似性高于与mβB2-cry或大鼠βB2-cry的相似性。
