克氏原螯虾中编码肌动蛋白同源物的cDNA序列。

Sequence of the cDNA encoding an actin homolog in the crayfish Procambarus clarkii.

作者信息

Kang W K, Naya Y

机构信息

Life Sciences Division, King's College London, UK.

出版信息

Gene. 1993 Nov 15;133(2):303-4. doi: 10.1016/0378-1119(93)90657-o.

DOI:10.1016/0378-1119(93)90657-o

PMID:8224920

Abstract

A cDNA library was constructed by using mRNAs purified from crayfish (Procambarus clarkii) muscle. Using a homology search of the nucleotide (nt) sequences, a clone of the library was found to encode a protein homologous to actin (Act). The insert fragment of this cDNA clone was 1072 nt in length. The amino acid sequence deduced from the nt sequence showed significant similarity to Act of various organisms as follows: 88.1% to Drosophila melanogaster, 88.2% to silk worm, 87.3% to brine shrimp, 86.3% to rat, and 86.3% to human (% identity).

摘要

利用从小龙虾（克氏原螯虾）肌肉中纯化的mRNA构建了一个cDNA文库。通过对核苷酸（nt）序列进行同源性搜索，发现该文库中的一个克隆编码一种与肌动蛋白（Act）同源的蛋白质。这个cDNA克隆的插入片段长度为1072 nt。从nt序列推导的氨基酸序列与各种生物的Act具有显著相似性，如下所示：与黑腹果蝇的相似性为88.1%，与家蚕的相似性为88.2%，与卤虫的相似性为87.3%，与大鼠的相似性为86.3%，与人类的相似性为86.3%（同一性百分比）。