DNA中烷基磷酸三酯的32P后标记分析。
32P-postlabelling analysis of alkylphosphotriesters in DNA.
作者信息
Saris C P, Damman S J, van den Ende A M, Westra J G, den Engelse L
机构信息
Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam.
出版信息
IARC Sci Publ. 1993(124):127-32.
PMID:8225475
Abstract
We have developed a highly sensitive 32P-postlabelling assay for the detection of alkylphosphotriesters in DNA using selective enzymatic DNA hydrolysis, sample preparation by C18 Sep-Pak solid phase extraction and product analysis by HPLC. The assay provides information on the quantity of the alkylphosphotriesters and gives some clues with respect to their identity.
摘要
我们开发了一种高灵敏度的³²P后标记分析法，用于检测DNA中的烷基磷酸三酯，该方法采用选择性酶促DNA水解、C18 Sep-Pak固相萃取进行样品制备以及HPLC进行产物分析。该分析法可提供有关烷基磷酸三酯数量的信息，并对其身份提供一些线索。
Zotero 插件