Saris C P, Damman S J, van den Ende A M, Westra J G, den Engelse L
Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam.
IARC Sci Publ. 1993(124):127-32.
We have developed a highly sensitive 32P-postlabelling assay for the detection of alkylphosphotriesters in DNA using selective enzymatic DNA hydrolysis, sample preparation by C18 Sep-Pak solid phase extraction and product analysis by HPLC. The assay provides information on the quantity of the alkylphosphotriesters and gives some clues with respect to their identity.
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