Schoket B
Department of Biochemistry, Johan Béla National Institute of Public Health, Budapest, Hungary.
IARC Sci Publ. 1993(124):341-7.
32P-Postlabelling has been used for biomonitoring occupational exposure to complex mixtures of polycyclic aromatic hydrocarbons in iron foundries, coke oven and aluminium plants and among roofers and surface-coating workers. Enhanced levels of aromatic DNA adducts have been detected in exposed populations in comparison to controls. Dose-related adduct formation has been found in iron foundry and coke-oven workers and roofers. The importance of longitudinal biomonitoring has been shown in two aluminium plants. Comparison between 32P-postlabelling and immunoassays revealed wide variations. DNA adduct levels obtained by the current methods should thus be regarded as relative values between individuals and control and exposure groups.
32P后标记法已用于生物监测铸造厂、炼焦炉、铝厂工人以及屋顶工和表面涂层工人职业接触多环芳烃复杂混合物的情况。与对照组相比，已在暴露人群中检测到芳香族DNA加合物水平升高。在铸造厂、炼焦炉工人和屋顶工中发现了剂量相关的加合物形成。在两家铝厂中已显示出纵向生物监测的重要性。32P后标记法与免疫测定法之间的比较显示出很大差异。因此，通过目前方法获得的DNA加合物水平应被视为个体与对照组和暴露组之间的相对值。
