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启动子间隔区和早期转录区的突变会增加葡萄球菌肠毒素A的表达。

Mutations in the promoter spacer region and early transcribed region increase expression of staphylococcal enterotoxin A.

作者信息

Borst D W, Betley M J

机构信息

Department of Bacteriology, University of Wisconsin-Madison 53706.

出版信息

Infect Immun. 1993 Dec;61(12):5421-5. doi: 10.1128/iai.61.12.5421-5425.1993.

DOI:10.1128/iai.61.12.5421-5425.1993
PMID:8225618
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC281336/
Abstract

The mechanism leading to increased production of staphylococcal enterotoxin type A (SEA) in mutant Staphylococcus aureus FRI722 compared within its wild-type parent strain, FRI100, was examined. Sequence analysis revealed two mutations in the upstream promoter region of FRI722 at nucleotides -28 and +3 with respect to the transcriptional initiation site at An sea translational fusion of the upstream region of FRI722 to the structural gene from FRI100 showed an increase in sea expression by Northern (RNA) analysis and in SEA production by Western (immunoblot) analysis. To independently evaluate the effect of each mutation, site-directed mutagenesis was done and revealed that each mutation was responsible for an increase in SEA production.

摘要

研究了突变型金黄色葡萄球菌FRI722与其野生型亲本菌株FRI100相比导致A型葡萄球菌肠毒素（SEA）产量增加的机制。序列分析显示，相对于转录起始位点A，FRI722上游启动子区域在核苷酸-28和+3处有两个突变。将FRI722上游区域与FRI100的结构基因进行sea翻译融合，通过Northern（RNA）分析显示sea表达增加，通过Western（免疫印迹）分析显示SEA产量增加。为了独立评估每个突变的影响，进行了定点诱变，结果表明每个突变都导致SEA产量增加。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f74/281336/d291b6c909c5/iai00024-0494-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f74/281336/87ac1b6c2dc7/iai00024-0492-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f74/281336/576c4bfe660d/iai00024-0493-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f74/281336/7e511bb3d869/iai00024-0494-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f74/281336/d291b6c909c5/iai00024-0494-c.jpg

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本文引用的文献

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Studies on transformation of Escherichia coli with plasmids.大肠杆菌质粒转化的研究。
J Mol Biol. 1983 Jun 5;166(4):557-80. doi: 10.1016/s0022-2836(83)80284-8.
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Compilation and analysis of Escherichia coli promoter DNA sequences.大肠杆菌启动子DNA序列的汇编与分析
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Induction of mutants of Staphylococcus aureus 100 with increased ability to produce enterotoxin A.诱导金黄色葡萄球菌100产生肠毒素A能力增强的突变体。
乙酸增加金黄色葡萄球菌噬菌体编码肠毒素 A 的表达。
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Staphylococcus aureus virulence expression is impaired by Lactococcus lactis in mixed cultures.在混合培养物中，金黄色葡萄球菌的毒力表达受到乳酸乳球菌的抑制。
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Transcription of the toxin genes present within the Staphylococcal phage phiSa3ms is intimately linked with the phage's life cycle.存在于金黄色葡萄球菌噬菌体phiSa3ms内的毒素基因转录与噬菌体的生命周期密切相关。
J Bacteriol. 2003 Dec;185(23):6841-51. doi: 10.1128/JB.185.23.6841-6851.2003.
6
Phage-associated differences in staphylococcal enterotoxin A gene (sea) expression correlate with sea allele class.噬菌体相关的葡萄球菌肠毒素A基因（sea）表达差异与sea等位基因类别相关。
Infect Immun. 1994 Jan;62(1):113-8. doi: 10.1128/iai.62.1.113-118.1994.
J Bacteriol. 1971 Apr;106(1):289-91. doi: 10.1128/jb.106.1.289-291.1971.
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Functional dissection of Escherichia coli promoters: information in the transcribed region is involved in late steps of the overall process.大肠杆菌启动子的功能剖析：转录区域中的信息参与了整个过程的后期步骤。
EMBO J. 1986 Nov;5(11):2995-3000. doi: 10.1002/j.1460-2075.1986.tb04597.x.
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Promoters of Escherichia coli: a hierarchy of in vivo strength indicates alternate structures.大肠杆菌的启动子：体内强度层次表明存在交替结构。
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Nucleotide sequence of the type A staphylococcal enterotoxin gene.A型葡萄球菌肠毒素基因的核苷酸序列。
J Bacteriol. 1988 Jan;170(1):34-41. doi: 10.1128/jb.170.1.34-41.1988.
7
Staphylococcal enterotoxin A is encoded by phage.葡萄球菌肠毒素A由噬菌体编码。
Science. 1985 Jul 12;229(4709):185-7. doi: 10.1126/science.3160112.
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J Mol Biol. 1989 Jun 20;207(4):749-56. doi: 10.1016/0022-2836(89)90241-6.
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Erythromycin-induced stabilization of ermA messenger RNA in Staphylococcus aureus and Bacillus subtilis.红霉素诱导金黄色葡萄球菌和枯草芽孢杆菌中ermA信使核糖核酸的稳定。
J Mol Biol. 1988 Oct 20;203(4):905-15. doi: 10.1016/0022-2836(88)90116-7.