大肠杆菌启动子DNA序列的汇编与分析
Compilation and analysis of Escherichia coli promoter DNA sequences.
作者信息
Hawley D K, McClure W R
出版信息
Nucleic Acids Res. 1983 Apr 25;11(8):2237-55. doi: 10.1093/nar/11.8.2237.
Abstract
The DNA sequence of 168 promoter regions (-50 to +10) for Escherichia coli RNA polymerase were compiled. The complete listing was divided into two groups depending upon whether or not the promoter had been defined by genetic (promoter mutations) or biochemical (5' end determination) criteria. A consensus promoter sequence based on homologies among 112 well-defined promoters was determined that was in substantial agreement with previous compilations. In addition, we have tabulated 98 promoter mutations. Nearly all of the altered base pairs in the mutants conform to the following general rule: down-mutations decrease homology and up-mutations increase homology to the consensus sequence.
摘要
汇编了168个大肠杆菌RNA聚合酶启动子区域（-50至+10）的DNA序列。根据启动子是否已通过遗传学（启动子突变）或生物化学（5'端确定）标准定义，将完整列表分为两组。基于112个明确界定的启动子之间的同源性确定了一个共有启动子序列，该序列与先前的汇编结果基本一致。此外，我们还列出了98个启动子突变。突变体中几乎所有改变的碱基对都符合以下一般规则：负突变降低与共有序列的同源性，正突变增加与共有序列的同源性。
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