Jiang L Q, Jorquera M, Streilein J W
Department of Microbiology and Immunology, University of Miami School of Medicine, Florida.
Invest Ophthalmol Vis Sci. 1993 Nov;34(12):3347-54.
Because immune rejection is likely to be a major barrier to successful retinal transplantation, it is important to determine whether immune privilege for allogeneic retinal grafts is a feature of the subretinal space and vitreous cavity.
Newborn neural retinas of C57BL/6 mice were implanted into the subretinal space, vitreous cavity, or subconjunctival space of eyes of adult BALB/c (disparate from C57BL/6 at major and minor histocompatibility loci). At postimplantation day 12, the recipients were evaluated for donor-specific delayed hypersensitivity and examined clinically and histologically for evidence of rejection.
Newborn neural retinal allografts in the subconjunctival space were destroyed by postimplantation day 12 and these recipients displayed intense donor-specific delayed hypersensitivity. By contrast, grafts in the subretinal space and vitreous cavity at postimplantation day 12 were found to be well differentiated and with no evidence of inflammation; these recipients failed to display donor-specific delayed hypersensitivity. Moreover, their spleens contained regulatory T cells that suppressed donor-specific delayed hypersensitivity in naive syngeneic recipients.
Allogeneic newborn neural retinal grafts implanted in the subretinal space and vitreous cavity experience immune privilege and induce deviant immune responses resembling anterior chamber associated immune deviation.
由于免疫排斥可能是视网膜移植成功的主要障碍，因此确定异体视网膜移植的免疫赦免是否是视网膜下间隙和玻璃体腔的一个特征非常重要。
将C57BL/6小鼠的新生神经视网膜植入成年BALB/c小鼠（在主要和次要组织相容性位点与C57BL/6不同）的视网膜下间隙、玻璃体腔或结膜下间隙。在植入后第12天，评估受体的供体特异性迟发型超敏反应，并进行临床和组织学检查以寻找排斥证据。
结膜下间隙的新生神经视网膜异体移植物在植入后第12天被破坏，这些受体表现出强烈的供体特异性迟发型超敏反应。相比之下，植入后第12天视网膜下间隙和玻璃体腔的移植物分化良好，没有炎症迹象；这些受体未表现出供体特异性迟发型超敏反应。此外，它们的脾脏含有调节性T细胞，可抑制同基因未致敏受体的供体特异性迟发型超敏反应。
植入视网膜下间隙和玻璃体腔的异体新生神经视网膜移植物具有免疫赦免，并诱导类似于前房相关免疫偏离的异常免疫反应。
