Troxel T R, Cruz L C, Ott R S, Kesler D J
Department of Animal Sciences, University of Illinois, Urbana 61801.
J Anim Sci. 1993 Oct;71(10):2579-85. doi: 10.2527/1993.71102579x.
In Exp. 1,101 suckled beef cows were administered two injections of prostaglandin F2 alpha (PGF2 alpha) 11 d apart. Cows in Group 1 received no additional treatment. Cows in Groups 2 and 3 received 250 micrograms of GnRH plus 47-h calf removal. Cows in Group 3 also received a norgestomet ear implant that was inserted 4 d after the first PGF2 alpha injection and left in situ for 8 d. The second PGF2 alpha injection was administered approximately 28 h before the time of norgestomet implant removal. The GnRH was administered approximately 30 h after the time of norgestomet implant removal (or 58 h after the second PGF2 alpha injection). Calf removal was the period from the time of implant removal to AI. All cows were artificially inseminated once 75 h after the second PGF2 alpha injection (47 h after implant removal). Reproductive status before the time of GnRH treatment (presynchronization) was determined by multiple sampling (2, 13, and 23 d before GnRH treatment) for blood progesterone concentrations (62% anestrous and 38% cyclic). The GnRH treatment increased (P < .01) the ovulation response and norgestomet reduced (P < .01) the incidence of short luteal phases in the presynchronization anestrous cows. Norgestomet treatment increased (P < .05) the timed breeding pregnancy rates for both presynchronization anestrous and cyclic cows. In Exp. 2, 174 suckled beef cows were administered Syncro-Mate B (which includes norgestomet). Cows in Group 1 received no additional treatment, whereas cows in Group 2 received 250 micrograms of GnRH (30 h after norgestomet implant removal).(ABSTRACT TRUNCATED AT 250 WORDS)
在实验1中,101头哺乳肉用母牛每隔11天接受两次前列腺素F2α(PGF2α)注射。第1组母牛未接受额外处理。第2组和第3组母牛接受250微克促性腺激素释放激素(GnRH)并进行47小时的犊牛隔离。第3组母牛还接受了炔诺孕酮耳部植入物,该植入物在首次PGF2α注射后4天插入,原位留存8天。第二次PGF2α注射在炔诺孕酮植入物取出前约28小时进行。GnRH在炔诺孕酮植入物取出后约30小时(或第二次PGF2α注射后58小时)给药。犊牛隔离期是从植入物取出到人工授精的时间段。所有母牛在第二次PGF2α注射后75小时(植入物取出后47小时)进行一次人工授精。通过多次采样(在GnRH处理前2、13和23天)测定血液孕酮浓度来确定GnRH处理前(预同步化)的生殖状态(62%处于乏情期,38%处于发情周期)。GnRH处理提高了(P <.01)排卵反应,炔诺孕酮降低了(P <.01)预同步化乏情期母牛短黄体期的发生率。炔诺孕酮处理提高了(P <.05)预同步化乏情期和发情周期母牛的定时配种妊娠率。在实验2中,174头哺乳肉用母牛接受了同步伴侣B(其中包括炔诺孕酮)。第1组母牛未接受额外处理,而第2组母牛接受250微克GnRH(在炔诺孕酮植入物取出后30小时)。(摘要截短至250字)