Kardalinou E, Eick S, Albig W, Doenecke D
Institut für Biochemie, Georg-August-Universität Göttingen, Germany.
J Cell Biochem. 1993 Aug;52(4):375-83. doi: 10.1002/jcb.240520402.
A cluster of human histone genes was found on three overlapping clones isolated from cosmid and bacteriophage libraries. These three overlapping segments of the human genome comprise genes coding for H3.1, an H2A pseudogene, and an H2B.1 gene downstream of the previously characterized H1.2 gene. The cosmid clone covers 30 kb upstream of the H1.2 gene and overlaps with two phage clones covering the core histone genes and the pseudogene. The same arrangement of an H3 gene, an H2A pseudogene and an H2B gene downstream of an H1 gene has been described within a mouse histone gene cluster [Yang et al.:J Biol Chem 262:17118-17125, 1987; Gruber et al.:Gene 95:303-304, 1990].
在从黏粒和噬菌体文库中分离出的三个重叠克隆上发现了一组人类组蛋白基因。人类基因组的这三个重叠片段包含编码H3.1的基因、一个H2A假基因以及位于先前鉴定的H1.2基因下游的一个H2B.1基因。黏粒克隆覆盖H1.2基因上游30 kb，并与两个覆盖核心组蛋白基因和假基因的噬菌体克隆重叠。在小鼠组蛋白基因簇中也描述了H1基因下游的H3基因、H2A假基因和H2B基因的相同排列方式[Yang等人：《生物化学杂志》262:17118 - 17125，1987年；Gruber等人：《基因》95:303 - 304，1990年]。
