使用在昆虫细胞中表达的重组病毒VP1抗原检测人细小病毒B19特异性IgM和IgG抗体，并通过检测抗体亲和力估计感染时间。

Detection of human parvovirus B19-specific IgM and IgG antibodies using a recombinant viral VP1 antigen expressed in insect cells and estimation of time of infection by testing for antibody avidity.

作者信息

Gray J J, Cohen B J, Desselberger U

机构信息

Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, UK.

出版信息

J Virol Methods. 1993 Sep;44(1):11-23. doi: 10.1016/0166-0934(93)90003-a.

DOI:10.1016/0166-0934(93)90003-a

PMID:8227275

Abstract

Sera from patients with symptoms of recent human parvovirus B19 (B19) infection were tested for B19-specific IgM in an immunofluorescence assay (IFA) using insect cells expressing B19 recombinant VP1 coat protein as an antigen. A highly significant correlation (P < 0.001) was found between titres obtained in the IgM IFA and the units obtained in an IgM antibody-capture RIA using plasma derived native B19 antigen. An IgG IFA using the recombinant antigen was performed on 57 sera and the antibody avidity determined. There was a highly significant correlation (P < 0.001) between the relative amounts of low avidity B19-specific IgG antibodies and time after onset of illness. This finding allows the detection of IgG to be used for diagnosing acute infection.

摘要

利用表达B19重组VP1衣壳蛋白的昆虫细胞作为抗原，通过免疫荧光试验（IFA）对近期感染人细小病毒B19（B19）且出现症状的患者血清进行B19特异性IgM检测。在IgM IFA中获得的滴度与使用血浆来源的天然B19抗原的IgM抗体捕获放射免疫分析（RIA）中获得的单位之间发现高度显著相关性（P < 0.001）。使用重组抗原对57份血清进行IgG IFA检测并测定抗体亲和力。低亲和力B19特异性IgG抗体的相对量与发病后的时间之间存在高度显著相关性（P < 0.001）。这一发现使得检测IgG可用于诊断急性感染。