Nonaka K, Sasaki Y, Yanagita K, Matsumoto T, Watanabe Y, Nakata M
Department of Pediatric Dentistry, Kyushu University Faculty of Dentistry, Fukuoka, Japan.
J Craniofac Genet Dev Biol. 1993 Jul-Sep;13(3):206-12.
Embryo transfer effect and intrauterine effect of the dam on prenatal development of the craniofacial complex of mice embryos were investigated with the use of embryo transfer and cephalostat. DDD strain embryos were transferred to the three strains of recipients (DDD, C57BL, and DBA). The cephalometric observation of newborn offspring developed from transferred embryos was performed just after parturition. Dorso-ventral craniofacial size of newborn offspring was calculated using values of X- and Y-coordinates on a dorsoventral cephalogram. Statistical analysis showed that a significant intergroup difference in craniofacial size between transferred and nontransferred groups as well as a significant inter-strain difference among those of the three strains of recipients were observed. Thus, it was disclosed that embryo transfer technique might retard the prenatal development of craniofacial complex of transferred embryo and that the three strains of recipients contributed unequally to the prenatal development of craniofacial complex of transferred embryo through each of their intrauterine environments as a prenatal maternal effect. These results indicated that the intrauterine environment of the recipient played an important role in the prenatal development of the craniofacial complex of the mice embryo.
利用胚胎移植和头颅固定器，研究了受体母鼠的胚胎移植效应和子宫内环境对小鼠胚胎颅面复合体产前发育的影响。将DDD品系胚胎移植到三种受体品系（DDD、C57BL和DBA）的母鼠体内。在分娩后立即对移植胚胎发育而成的新生后代进行头颅测量观察。利用背腹位头颅X线片上X和Y坐标值计算新生后代的背腹位颅面大小。统计分析表明，移植组与未移植组之间颅面大小存在显著的组间差异，三种受体品系之间也存在显著的品系间差异。因此，研究发现胚胎移植技术可能会延缓移植胚胎颅面复合体的产前发育，并且这三种受体品系通过其各自的子宫内环境（作为产前母体效应）对移植胚胎颅面复合体的产前发育贡献不均等。这些结果表明，受体的子宫内环境在小鼠胚胎颅面复合体的产前发育中起着重要作用。
