Tregear R, Oiwa K, Chaen S, Sugi H
Department of Physiology, School of Medicine, Teikyo University, Tokyo, Japan.
J Muscle Res Cell Motil. 1993 Aug;14(4):412-5. doi: 10.1007/BF00121292.
We have succeeded in controlling the sliding movement of myosin-coated magnetizable beads on actin cables in Nitellopsis cells by the inhomogeneous magnetic field adjacent to a small, strong permanent magnet. The relation between magnetic force acting on the bead and the bead velocity was, in many respects, similar to that obtained from the same system by the use of centrifugal force (Oiwa et al., 1990). In particular, force favouring the motion (negative load) had little effect on the velocity until it was sufficient to pull the bead off the actin, whereas a relatively small positive load caused a reduction in velocity to a plateau value. Although the present method does not allow a good control of force direction, it demonstrates the promise of magnetic force in studying in vitro motility.
我们已成功通过靠近一个小的强永磁体的非均匀磁场，控制了丽藻细胞中肌球蛋白包被的可磁化珠子在肌动蛋白丝上的滑动运动。作用于珠子的磁力与珠子速度之间的关系在许多方面，类似于通过使用离心力从同一系统获得的关系（大岩等人，1990年）。特别是，有利于运动的力（负负载）在足以将珠子从肌动蛋白上拉下来之前，对速度几乎没有影响，而相对较小的正负载会导致速度降低至平稳值。尽管目前的方法无法很好地控制力的方向，但它证明了磁力在体外运动性研究中的前景。
