Mooney R D, Nikoletseas M M, King T D, Savage S V, Huang X
Department of Anatomy, Medical College of Ohio, Toledo 43699.
J Comp Neurol. 1993 Sep 8;335(2):214-27. doi: 10.1002/cne.903350206.
Extracellular and intracellular recording, receptive field mapping, and intracellular HRP injection techniques were used to define the morphological classes of cells in the deep laminae of the hamster's superior colliculus and to determine whether there are any correlations between the structural and functional characteristics of these neurons. A total of 110 neurons were characterized and reconstructed. Of these, 23.6% (N = 26) were visual, 60% (N = 66) were somatosensory, 0.9% (N = 1) were bimodal (visual-somatosensory), and 15.4% (N = 17) were unresponsive. Of the somatosensory neurons, 72.7% (N = 48) were low threshold, 4.5% (N = 3) had a wide dynamic range, 9.1% (N = 6) responded only to noxious stimulation, and 13.6% (N = 9) had complex somatosensory receptive fields. Deep layer cells were divided into eight morphological classes. These classes were multipolar cells (26.4%, N = 29), bipolar cells (9.1%, N = 10), widefield vertical cells (7.3%, N = 8), horizontal cells (13.6%, N = 15), stellate cells (10.9%, N = 12), ventrally directed cells (5.5%, N = 6), sparse radial cells (17.3%, N = 19), and small sparse radial cells (6.4%, N = 7). Four cells (3.6%) did not fit into this classification scheme. Univariate and multivariate analyses of variance of properties such as soma area, number of branch points, total dendritic length, and volume and orientation of dendritic arbor indicated that these classes were significantly different. However, chi 2 analysis and multivariate analysis of variance indicated no significant relationships between morphological class and either laminar location or receptive field type. There was a significant positive relationship between the possession of dendrites that extended into the superficial laminae and visual responsivity.
采用细胞外和细胞内记录、感受野映射以及细胞内辣根过氧化物酶注射技术，来确定仓鼠上丘深层细胞的形态类别，并确定这些神经元的结构和功能特征之间是否存在任何相关性。总共对110个神经元进行了特征描述和重建。其中，23.6%（N = 26）为视觉神经元，60%（N = 66）为躯体感觉神经元，0.9%（N = 1）为双模态（视觉 - 躯体感觉）神经元，15.4%（N = 17）无反应。在躯体感觉神经元中，72.7%（N = 48）为低阈值神经元，4.5%（N = 3）具有宽动态范围，9.1%（N = 6）仅对有害刺激有反应，13.6%（N = 9）具有复杂的躯体感觉感受野。深层细胞分为八个形态类别。这些类别为多极细胞（26.4%，N = 29）、双极细胞（9.1%，N = 10）、宽视野垂直细胞（7.3%，N = 8）、水平细胞（13.6%，N = 15）、星状细胞（10.9%，N = 12）、腹侧定向细胞（5.5%，N = 6）、稀疏放射状细胞（17.3%，N = 19）和小稀疏放射状细胞（6.4%，N = 7）。四个细胞（3.6%）不符合此分类方案。对诸如胞体面积、分支点数、总树突长度以及树突分支的体积和方向等属性进行单变量和多变量方差分析表明，这些类别存在显著差异。然而，卡方分析和多变量方差分析表明，形态类别与层位位置或感受野类型之间均无显著关系。延伸至浅层的树突的存在与视觉反应性之间存在显著正相关。
