Rigas B
J Clin Gastroenterol. 1993 Oct;17(3):184-6.
Molecular biology has revolutionized our understanding of the life of the cell. The development of concepts has been accompanied by the development of new technologies, many of which have clinical applicability. This new armamentarium includes methods for detection and amplification of minuscule amounts of DNA, automated methods for DNA and RNA sequencing, the ability to express a gene in cultured cells and harvest its product, approaches to identify genes whose product is unknown, methods for a detailed understanding of the regulation of gene expression, and innovative methods for gene therapy. These developments have already had an impact on gastroenterology, exemplified by the identification of hepatitis viruses and the agent causing Whipple's disease, development of a recombinant vaccine for hepatitis B, and isolation of the cystic fibrosis gene, which will lead to the diagnosis and treatment of this disease. This editorial outlines these recent changes in the conceptual and practical framework of gastroenterology.
分子生物学彻底改变了我们对细胞生命的理解。概念的发展伴随着新技术的发展，其中许多技术具有临床适用性。这一新型手段包括检测和扩增微量DNA的方法、DNA和RNA测序的自动化方法、在培养细胞中表达基因并收获其产物的能力、鉴定产物未知基因的方法、详细了解基因表达调控的方法以及基因治疗的创新方法。这些进展已经对胃肠病学产生了影响，例如肝炎病毒和导致惠普尔病的病原体的鉴定、乙型肝炎重组疫苗的研发以及囊性纤维化基因的分离，这将带来该疾病的诊断和治疗。这篇社论概述了胃肠病学概念和实践框架中的这些最新变化。
