宿主组蛋白与犰狳源麻风分枝杆菌之间的关系。
Relationship between host histones and armadillo-derived Mycobacterium leprae.
作者信息
Pessolani M C, Hunter S W, Brennan P J
机构信息
Department of Microbiology, Colorado State University, Fort Collins 80523.
出版信息
Int J Lepr Other Mycobact Dis. 1993 Sep;61(3):381-8.
A major protein previously recognized as being primarily associated with the cell walls of Mycobacterium leprae, major wall protein (MWP), is now identified as histoprotein H2b based on N-terminal amino-acid sequencing, electrophoretic comparisons, and several other properties. An avid association between several host/armadillo-derived histones and M. leprae was demonstrated. Since such armadillo-derived M. leprae are the basis of several ongoing vaccine trials, a simple procedure that permits the prompt solubilization and quantification of histones in M. leprae preparations is described. The quantity of histones associated with M. leprae is significant, ranging from 0.6 to 4.8 micrograms of histoprotein H2b per mg of bacteria.
一种先前被认为主要与麻风分枝杆菌细胞壁相关的主要蛋白质，即主要壁蛋白（MWP），现在基于N端氨基酸测序、电泳比较及其他几种特性被鉴定为组织蛋白H2b。已证明几种宿主/犰狳来源的组蛋白与麻风分枝杆菌之间存在紧密关联。由于这种犰狳来源的麻风分枝杆菌是正在进行的几项疫苗试验的基础，本文描述了一种简单的程序，可快速溶解和定量麻风分枝杆菌制剂中的组蛋白。与麻风分枝杆菌相关的组蛋白数量可观，每毫克细菌中组织蛋白H2b的含量在0.6至4.8微克之间。
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