酪氨酸酶在聚丙烯酰胺凝胶中的固定化。

Immobilization of tyrosinase within polyacrylamide gels.

作者信息

Schiller J G, Liu C C

出版信息

Biotechnol Bioeng. 1976 Oct;18(10):1405-12. doi: 10.1002/bit.260181007.

Abstract

The enzyme tyrosinase (E.C. 1.14.18.1)has been immobilized in a polyacrylamide gel and intermittently assayed for enzyme activity over a period of 19 days using phenol as the substrate. The results of these studies indicate that the immobilized enzyme could be incorporated into a system to detect phenol and related compounds that are found in industrial effluents and as surface water contaminants.

摘要

酪氨酸酶（E.C. 1.14.18.1）已被固定在聚丙烯酰胺凝胶中，并以苯酚为底物，在19天的时间内间歇性地检测其酶活性。这些研究结果表明，固定化酶可被纳入一个系统，用于检测工业废水中以及作为地表水污染物存在的苯酚和相关化合物。