在大肠杆菌B的吡哆醇营养缺陷型培养物中5-羟基-L-正缬氨酸的鉴定。
The identification of 5-hydroxy-L-norvaline in cultures of pyridoxine auxotrophs of Escherichia coli B.
作者信息
Hill R E, White R L, Smith K C
机构信息
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
出版信息
J Nat Prod. 1993 Aug;56(8):1246-54. doi: 10.1021/np50098a007.
Gc-ms and hplc have been employed to identify 5-hydroxy-L-norvaline (L-2-amino-5-hydroxypentanoic acid) in the culture fluids of pyridoxine-starved cultures of two pyridoxine auxotrophs of Escherichia coli B. The production of 5-hydroxynorvaline is shown to be secondary to pyridoxine starvation of the cultures, and the quantities of 5-hydroxynorvaline are similar to those of the common protein amino acids present in these cultures. This is the first time that 5-hydroxynorvaline has been identified as a product of bacterial metabolism.
气相色谱-质谱联用仪（Gc-ms）和高效液相色谱（hplc）已被用于鉴定大肠杆菌B的两种吡哆醇营养缺陷型菌株在吡哆醇饥饿培养物的培养液中的5-羟基-L-正缬氨酸（L-2-氨基-5-羟基戊酸）。结果表明，5-羟基正缬氨酸的产生是培养物吡哆醇饥饿的继发效应，且其含量与这些培养物中常见的蛋白质氨基酸含量相似。这是首次将5-羟基正缬氨酸鉴定为细菌代谢产物。
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