Forsberg M, Linde-Forsberg C, Karlsson A, Carlsson M A
Department of Clinical Chemistry, College of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
J Reprod Fertil Suppl. 1993;47:127-32.
The Amerlite immunoassay system was evaluated for direct measurement of progesterone and oestradiol in canine plasma. The progesterone assay was evaluated without modification. To increase the sensitivity of the oestradiol assay, the horseradish peroxidase-labelled tracer was diluted 1:2 and the antiserum 1:10. Subsequently, the standards supplied in the kit were altered to produce a new standard curve. The dilution curves of canine plasma samples with high progesterone and oestradiol contents were parallel with the standard curves based on human serum. The relation between measurements of both hormones in canine plasma using established extraction methods as references and the Amerlite procedures were highly comparable, resulting in the linear regression equations y = 0.91x + 1.3 (progesterone, n = 100) and y = 0.79x + 0.9 (oestradiol, n = 84). However, for oestradiol the closest relation to the reference method was achieved when the incubation period was extended to 24 h at 4 degrees C. When measuring oestradiol and progesterone in canine sera during the oestrous cycle the hormone patterns were not influenced by the methods applied.
对Amerlite免疫分析系统进行了评估，以直接测定犬血浆中的孕酮和雌二醇。孕酮分析未经修改进行评估。为提高雌二醇分析的灵敏度，将辣根过氧化物酶标记的示踪剂稀释1:2，抗血清稀释1:10。随后，改变试剂盒中提供的标准品以生成新的标准曲线。高孕酮和雌二醇含量的犬血浆样品的稀释曲线与基于人血清的标准曲线平行。以既定提取方法为参考，使用Amerlite方法测定犬血浆中两种激素的结果具有高度可比性，得出线性回归方程y = 0.91x + 1.3（孕酮，n = 100）和y = 0.79x + 0.9（雌二醇，n = 84）。然而，对于雌二醇，当在4℃下将孵育期延长至24小时时，与参考方法的关系最为接近。在发情周期中测定犬血清中的雌二醇和孕酮时，激素模式不受所用方法的影响。
