Rodriguez-Martinez H, Ekwall H, Linde-Forsberg C
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
J Reprod Fertil Suppl. 1993;47:279-85.
Dog spermatozoa from fresh ejaculates and after freezing-thawing were air-dried on to grids and subjected to X-ray microanalysis or examined by scanning and transmission electron microscopy. The spermatozoa subjected to freezing-thawing presented major changes in their morphology, which included the loss of acrosomal contents, seen both by the swelling and rarefaction of the acrosome and the loss of electron-dense acrosomal material. In many cells the acrosomal damage, including the equatorial region, was conspicuous, with vesiculization of the acrosomal membranes. The plasmalemma remained, in most cases, apparently intact. X-ray microanalysis of the sperm aliquots showed a significant decrease in the amount of most selected elements after freezing-thawing. The extent to which concentrations of phosphorus and sulfur decreased in the sperm head region suggested that major changes in the chromatin occurred during the freeze-thaw process.
将新鲜射精及冻融后的犬精子风干于网格上，进行X射线微分析，或通过扫描电子显微镜和透射电子显微镜进行检查。经历冻融的精子在形态上出现了重大变化，包括顶体内容物的丢失，这可通过顶体的肿胀和稀疏以及电子致密顶体物质的丢失看出。在许多细胞中，包括赤道区在内的顶体损伤很明显，顶体膜出现囊泡化。在大多数情况下，质膜似乎保持完整。对精子样本进行的X射线微分析显示，冻融后大多数选定元素的含量显著下降。精子头部区域磷和硫浓度下降的程度表明，在冻融过程中染色质发生了重大变化。
