Dobrinski I, Lulai C, Barth A D, Post K
Department of Herd Medicine and Theriogenology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
J Reprod Fertil Suppl. 1993;47:291-6.
Ejaculates were collected from 17 dogs. After initial evaluation the ejaculates were split into four equal aliquots and diluted with either Triladyl, Pipes, IMV Universal or Tris-fructose-citric acid extender, all containing egg yolk and glycerol. The extended semen was frozen in 0.5 ml straws at a slow, intermediate or fast freezing rate. After thawing, the percentage of progressively motile spermatozoa and the velocity of forward motion was evaluated. There was no difference between the extenders with regard to progressive motility. The slow, intermediate and fast freezing rates resulted in the highest, intermediate and lowest motility values, respectively, regardless of extender. All four extenders were equally suitable for freezing canine semen at a slow freezing rate.
从17只狗身上采集精液。初步评估后，将精液分成四个等量的部分，并用含有蛋黄和甘油的Triladyl、Pipes、IMV通用或三羟甲基氨基甲烷 - 果糖 - 柠檬酸稀释液进行稀释。将稀释后的精液以慢速、中速或快速冷冻速率冷冻在0.5毫升的细管中。解冻后，评估前进运动精子的百分比和向前运动的速度。在前进运动能力方面，不同稀释液之间没有差异。无论使用哪种稀释液，慢速、中速和快速冷冻速率分别导致最高、中等和最低的运动能力值。所有四种稀释液同样适合以慢速冷冻速率冷冻犬精液。
