Valay J G, Simon M, Faye G
Institut Curie-Biologie, Centre Universitaire, Orsay, France.
J Mol Biol. 1993 Nov 20;234(2):307-10. doi: 10.1006/jmbi.1993.1587.
Kin28 is an essential serine-threonine kinase of Saccharomyces cerevisiae. Multicopies of a novel cyclin gene, CCL1, are able to suppress the thermosensitivity of two kin28-ts mutants. The CCL1 gene is not cyclically transcribed, yet its product is also essential for cell proliferation. Furthermore, when overexpressed under high expression promoter, Cell is able to replace G1 function of Cln cyclins. Cell and Kin28 are physically associated in vivo. Therefore, like p34CDC28/cdc2, Kin28 may be a cyclin dependent kinase which is required for cell proliferation.
Kin28是酿酒酵母中一种必需的丝氨酸-苏氨酸激酶。一个新的细胞周期蛋白基因CCL1的多拷贝能够抑制两个kin28-ts突变体的温度敏感性。CCL1基因不是周期性转录的，但其产物对细胞增殖也是必需的。此外，当在高表达启动子下过表达时，Cell能够取代Cln细胞周期蛋白的G1功能。Cell和Kin28在体内存在物理关联。因此，与p34CDC28/cdc2一样，Kin28可能是细胞增殖所需的一种细胞周期蛋白依赖性激酶。
