Carey J, Combatti N, Lewis D E, Lawson C L
Chemistry Department, Princeton University, NJ 08544-1009.
J Mol Biol. 1993 Nov 20;234(2):496-8. doi: 10.1006/jmbi.1993.1602.
Cocrystals of a 2:1 complex of trp repressor dimers with a DNA duplex containing a single, central operator half-site sequence are described. Crystals with different morphologies grew under diverse crystallization conditions within days to weeks by hanging-drop vapor-diffusion. Twinned rods split along their longitudinal cleft produce single crystals with space group C2 and unit cell dimensions a = 112.34 A, b = 90.16 A, c = 58.65 A and beta = 113.92 degrees. The crystals diffract to 2.4 A and are thus suitable for structural analysis by X-ray diffraction. Several heavy-atom derivative cocrystals have been obtained with iodouridine-substituted DNAs.
本文描述了色氨酸阻遏物二聚体与包含单个中央操纵子半位点序列的DNA双链体形成的2:1复合物的共晶体。通过悬滴气相扩散法，在不同的结晶条件下，数天到数周内生长出具有不同形态的晶体。沿其纵向裂隙分裂的孪晶棒产生空间群为C2的单晶，晶胞参数为a = 112.34 Å，b = 90.16 Å，c = 58.65 Å，β = 113.92°。这些晶体的衍射分辨率达到2.4 Å，因此适用于X射线衍射结构分析。已获得了几种用碘尿苷取代的DNA的重原子衍生物共晶体。
