Bannerman P G, Pleasure D
Children's Hospital of Philadelphia, Pennsylvania 19104.
J Neurosci Res. 1993 Sep 1;36(1):46-57. doi: 10.1002/jnr.490360106.
Nascent neural crest cells derived from explanted E12 embryonic rat caudal neural tubes were used as an assay system to investigate the effects of fibroblast growth factors on neural crest cell (NCC) survival, proliferation, migration, and differentiation. In vitro and in vivo all NCC express low affinity nerve growth factor receptors (p75-LNGFR), whereas a subpopulation of NCC expresses the carbohydrate epitope recognized by the monoclonal antibody HNK-1 (Bannerman and Pleasure, manuscript in preparation). Both acidic and basic fibroblast growth factor (FGF) promoted the survival of proportionally greater numbers of p75-LNGF+/HNK-1- than P75-LNGFR+/HNK-1+ NCC. An as yet uncharacterized factor present in neural tube-conditioned medium was also required for NCC survival. Mitosis was frequent in those NCC closest to the neural tube, less so as the cells migrated away. Neither basic nor acidic fibroblast growth factor (FGF) influenced rates of NCC mitosis in either of these locations, nor did these FGFs alter the rate at which nascent NCC migrated away from the neural tube. However, acidic and basic FGFs did delay the differentiation of neural crest derived neurons in the cultures. FGF is abundant in the embryonic rat neural crest outgrowth zone, and the present study strongly supports an essential role for FGF in early development of the mammalian neural crest.
从E12期大鼠胚胎尾神经管外植体中分离出的新生神经嵴细胞被用作检测系统，以研究成纤维细胞生长因子对神经嵴细胞（NCC）存活、增殖、迁移和分化的影响。在体外和体内，所有NCC均表达低亲和力神经生长因子受体（p75-LNGFR），而NCC的一个亚群表达单克隆抗体HNK-1识别的碳水化合物表位（Bannerman和Pleasure，正在准备的手稿）。酸性和碱性成纤维细胞生长因子（FGF）均可促进比例上更多的p75-LNGF+/HNK-1-而非P75-LNGFR+/HNK-1+ NCC的存活。神经管条件培养基中存在的一种尚未鉴定的因子对于NCC的存活也是必需的。有丝分裂在最靠近神经管的那些NCC中很频繁，随着细胞迁移离开则频率降低。碱性或酸性成纤维细胞生长因子（FGF）在这两个位置均不影响NCC的有丝分裂速率，这些FGF也不会改变新生NCC从神经管迁移离开的速率。然而，酸性和碱性FGF确实延迟了培养物中神经嵴衍生神经元的分化。FGF在胚胎大鼠神经嵴生长区中含量丰富，本研究有力地支持了FGF在哺乳动物神经嵴早期发育中的重要作用。
