Thomas P T, House R V, Bhargava H N
Life Sciences Research, IIT Research Institute, Chicago, Illinois 60616.
Life Sci. 1993;53(18):1417-27. doi: 10.1016/0024-3205(93)90584-p.
Phencyclidine hydrochloride (PCP) was tested for its ability to alter a variety of immune effector and regulatory functions in vitro. B6C3F1 murine splenic lymphocytes or elicited peritoneal macrophages were cultured in vitro with medium only or medium containing 10(-10)-10(-4) M PCP. Macrophages cultured with or without PCP were stimulated with lipopolysaccharide, and production of interleukin 6 (IL-6) and tumor necrosis factor (TNF) was assessed by bioassay. Cytotoxic T-cell effector function was determined following 5-day lymphocyte co-culture with tumor stimulator cells in the presence of PCP. In addition, the ability of T-lymphocytes to produce specific immunoregulatory cytokines IL-2 and IL-4 in the presence of PCP was quantitated by bioassay. B-lymphocyte function was determined by quantitating lymphocyte proliferation following stimulation with anti-IgM antibody and murine IL-4. Natural immunity was assessed by culturing lymphocytes with or without PCP for 24 h, then quantitating basal and IL-2 augmented natural killer (NK) cell activity. In the absence of effects on cell viability, significant suppression of IL-2 production by T-cells was noted at pharmacologically relevant PCP concentrations (1 microM). In vitro concentrations of 10 microM suppressed the generation of specifically sensitized cytotoxic T-cells. In addition, PCP significantly suppressed both IL-2-augmented NK function as well as B-lymphocyte proliferation. By comparison, macrophage IL-6 production was not affected by any concentration of PCP examined in this study.
对盐酸苯环利定（PCP）改变多种体外免疫效应和调节功能的能力进行了测试。将B6C3F1小鼠脾淋巴细胞或诱导的腹腔巨噬细胞在仅含培养基或含10⁻¹⁰ - 10⁻⁴ M PCP的培养基中进行体外培养。用脂多糖刺激培养有或没有PCP的巨噬细胞，并通过生物测定评估白细胞介素6（IL - 6）和肿瘤坏死因子（TNF）的产生。在PCP存在的情况下，与肿瘤刺激细胞进行5天淋巴细胞共培养后，测定细胞毒性T细胞效应功能。此外，通过生物测定对在PCP存在下T淋巴细胞产生特异性免疫调节细胞因子IL - 2和IL - 4的能力进行定量。通过用抗IgM抗体和小鼠IL - 4刺激后定量淋巴细胞增殖来测定B淋巴细胞功能。通过在有或没有PCP的情况下培养淋巴细胞24小时，然后定量基础和IL - 2增强的自然杀伤（NK）细胞活性来评估天然免疫。在对细胞活力无影响的情况下，在药理学相关的PCP浓度（1 microM）下，观察到T细胞产生IL - 2受到显著抑制。体外浓度为10 microM时抑制了特异性致敏细胞毒性T细胞的生成。此外，PCP显著抑制了IL - 2增强的NK功能以及B淋巴细胞增殖。相比之下，本研究中检测的任何浓度的PCP均未影响巨噬细胞IL - 6的产生。
