Fandeur T, Vazeux G, Mercereau-Puijalon O
Institut Pasteur de Guyane, Laboratoire de Parasitologie Moléculaire, Cayenne, French Guiana.
Mol Biochem Parasitol. 1993 Aug;60(2):241-8. doi: 10.1016/0166-6851(93)90135-k.
The Palo Alto strain of Plasmodium falciparum is highly virulent for the Saimiri sciureus monkey. We have observed that these parasites do not express the Ring-infected erythrocyte surface antigene (RESA) gene. Immunoblots indicated that the Pf155/RESA protein was absent. The RESA mRNA could not be detected. Polymerase chain reaction and Southern blot analysis demonstrated that this lack of expression is due to gene rearrangements. The majority of the Palo Alto parasites have a deletion of the entire RESA gene, whereas in a minor fraction the RESA sequences remain detectable, but the 5' miniexon 1 is inverted. These data show that the RESA protein is dispensable for in vivo parasite growth, at least in Saimiri monkeys.
恶性疟原虫的帕洛阿尔托株对松鼠猴具有高度毒性。我们观察到这些寄生虫不表达环状感染红细胞表面抗原（RESA）基因。免疫印迹表明不存在Pf155/RESA蛋白。无法检测到RESA mRNA。聚合酶链反应和Southern印迹分析表明，这种表达缺失是由于基因重排所致。大多数帕洛阿尔托疟原虫的整个RESA基因发生缺失，而在一小部分中仍可检测到RESA序列，但5'小外显子1发生了倒置。这些数据表明，RESA蛋白对于体内寄生虫的生长并非必需，至少在松鼠猴中是这样。
