Cole D G, Chinn S W, Wedaman K P, Hall K, Vuong T, Scholey J M
Section of Molecular and Cellular Biology, University of California, Davis 95616.
Nature. 1993 Nov 18;366(6452):268-70. doi: 10.1038/366268a0.
Kinesin heavy chain and kinesin-related polypeptides (KRPs) comprise a family of motor proteins with diverse intracellular transport functions. Using pan-kinesin peptide antibodies that react with these proteins, we have previously purified from sea urchin eggs a trimeric microtubule-binding and bundling protein, KRP (85/95) (ref. 8) comprising subunits of M(r) 115,000 (115K), 95K and 85K. We report here that kinesin-related genes encode the 85K and 95K subunits, and that the protein can be immunoprecipitated from cytosol as a trimeric complex using an 85K monoclonal antibody. We also find that purified KRP(85/95) directs movements towards the 'plus' ends of microtubules. To our knowledge, this protein is the first kinesin-related motor to be purified from its natural host cell in a native multimeric state.
驱动蛋白重链和驱动蛋白相关多肽（KRPs）构成了一个具有多种细胞内运输功能的运动蛋白家族。利用与这些蛋白质发生反应的泛驱动蛋白肽抗体，我们之前从海胆卵中纯化出了一种三聚体微管结合和捆绑蛋白，即KRP（85/95）（参考文献8），它由分子量为115,000（115K）、95K和85K的亚基组成。我们在此报告，驱动蛋白相关基因编码85K和95K亚基，并且该蛋白可以使用85K单克隆抗体从胞质溶胶中作为三聚体复合物进行免疫沉淀。我们还发现，纯化的KRP（85/95）能引导物质向微管的“正”端移动。据我们所知，这种蛋白是首个以天然多聚体状态从其天然宿主细胞中纯化出来的驱动蛋白相关运动蛋白。
