Vigot R, Billard J M, Batini C
Laboratoire de Physiologie de la Motricité, CNRS, Université Pierre et Marie Curie, CHU Pitié-Salpêtrière, Paris, France.
Neurosci Res. 1993 Aug;17(3):249-55. doi: 10.1016/0168-0102(93)90052-r.
GABA agonists were iontophoretically applied to Purkinje cells (PCs) of the cerebellar cortex and to neurons of the cerebellar nuclei (NCNs) in normal and in climbing fibre (CF) deafferented cerebella of rat. The experiments were performed one and three months after CF deafferentation obtained by total inferior olive destruction with 3-acetylpyridine. All control PCs were dose-dependently inhibited by GABA and muscimol and nearly all by baclofen. After CF deafferentation, the number of PCs sensitive to muscimol remained the same but the number sensitive to baclofen was greatly reduced one month later and almost absent after three months. The number of NCNs inhibited by GABA was slightly reduced one month after deafferentation compared to controls, but reduced to less than half three months after. Thus CF deafferentation of the PC leads to changes in postsynaptic sensitivity to GABA, the presumed inhibitory neurotransmitter, affecting GABAA receptors of the NCNs and GABAB receptors of the PCs.
将γ-氨基丁酸（GABA）激动剂通过离子电渗法施加于正常大鼠以及通过用3-乙酰吡啶破坏全部下橄榄核而导致攀缘纤维（CF）传入阻滞的大鼠小脑皮质浦肯野细胞（PCs）和小脑核神经元（NCNs）。实验在通过3-乙酰吡啶完全破坏下橄榄核导致CF传入阻滞1个月和3个月后进行。所有对照PCs均受到GABA、蝇蕈醇的剂量依赖性抑制，几乎所有PCs也受到巴氯芬的抑制。CF传入阻滞1个月后，对蝇蕈醇敏感的PCs数量保持不变，但对巴氯芬敏感的PCs数量大幅减少，3个月后几乎消失。与对照组相比，去传入神经1个月后，受GABA抑制的NCNs数量略有减少，但3个月后减少至不到一半。因此，PCs的CF传入阻滞导致对GABA（一种假定的抑制性神经递质）的突触后敏感性发生变化，影响NCNs的GABAA受体和PCs的GABAB受体。
